BOK基因敲除Hela细胞
货号:
EDJ-KQ52730
物种:
人
细胞名称:
HeLa
基因名称:
BOK
基因ID:
666
规格:
1×10⁶cells
BOK基因敲除细胞Hela是由艾迪基因优化的CRISPR/Cas9编辑而成,采用Sanger测序法验证敲除,保证单克隆,活性良好。
| 货号 | EDJ-KQ52730 |
|---|---|
| 产品名称 | BOK Knockout Hela Cell Line |
| 细胞 | Hela |
| Cellosaurus ID | CVCL_0030 |
| 细胞别名 | HELA, Hela, He La, He-La, HeLa-CCL2, Henrietta Lacks cells, Helacyton gartleri |
| 基因 | BOK |
| 基因ID | |
| 基因别名 | BCL2L9|BOKL |
| 摘要 |
The protein encoded by this gene belongs to the BCL2 family, members of which form homo- or heterodimers, and act as anti- or proapoptotic regulators that are involved in a wide variety of cellular processes. Studies in rat show that this protein has restricted expression in reproductive tissues, interacts strongly with some antiapoptotic BCL2 proteins, not at all with proapoptotic BCL2 proteins, and induces apoptosis in transfected cells. Thus, this protein represents a proapoptotic member of the BCL2 family. [provided by RefSeq, Sep 2011]
|
| 癌症类型 | Cervical Carcinoma |
| 细胞形态 | Adherent |
| 传代比率 | 1/5, 2days |
| 完全培养基 | MEM + 10% FBS |
| 冻存培养基 | 70%完全培养基+ 20% FBS+ 10% DMSO |
* 仅供科研使用,不适用于人体或动物,包括临床、治疗或诊断用途。
| Loci | 送检细胞STR信息 送检细胞名: HeLa | 细胞库细胞STR信息 细胞库细胞名: HeLa | ||
| Allele1 | Allele2 | Allele1 | Allele2 | |
| Amelogenin | X | X | ||
| CSF1PO | 9 | 10 | 9 | 10 |
| D1S1656 | 12 | 15 | 12 | 15 |
| D2S1338 | 17 | 17 | ||
| D3S1358 | 15 | 18 | 15 | 18 |
| D5S818 | 11 | 12 | 11 | 12 |
| D6S1043 | 18 | 18 | ||
| D7S820 | 8 | 12 | 8 | 12 |
| D8S1179 | 12 | 13 | 12 | 13 |
| D12S391 | 20 | 25 | 20 | 25 |
| D13S317 | 12 | 14 | 12 | 14 |
| D16S539 | 9 | 10 | 9 | 10 |
| D18S51 | 16 | 16 | ||
| D19S433 | 13 | 14 | 13 | 14 |
| D21S11 | 27 | 28 | 27 | 28 |
| FGA | 18 | 21 | 18 | 21 |
| Penta D | 8 | 15 | 8 | 15 |
| Penta E | 7 | 17 | 7 | 17 |
| TPOX | 8 | 12 | 8 | 12 |
| VWA | 16 | 18 | 16 | 18 |
* 该细胞系与收录于ATCC, DSMZ, JCRB 和 RIKEN数据库的细胞系STR数据匹配。
结论:该细胞 STR 鉴定正确。
结论:该细胞 STR 鉴定正确。
* 研究用途免责声明:本内容基于公开的研究数据、生物信息学资源及计算分析生成,仅供研究参考。
相关研究文献
使用邻近标记法识别 Bok Interactome。
IF=4.3
Frontiers in cell and developmental biology
该敲除模型可能有助于:
- 通过邻近标记法绘制蛋白质-蛋白质相互作用图谱和Bok相互作用组图谱;
- 研究Bok在细胞凋亡和线粒体动力学中的作用;
- 对相互作用组研究中鉴定的候选结合蛋白进行功能验证;
- 研究人类癌细胞系中细胞死亡通路机制;
- 比较分析Bok与其他Bcl-2家族成员的功能。