说明书 
艾迪基因基于自主研发的EditX™基因编辑平台，采用优化升级的CRISPR/Cas9系统，设计科学的CCR6基因敲除方案。通过RNP法、瞬转质粒法或慢病毒法将CRISPR/Cas9编辑体系递送到HEPG2细胞中，然后经过真核抗性标记筛选出阳性细胞池，再使用3D单细胞打印技术挑选单克隆细胞，最后通过基因组测序验证，检测合格后对CCR6基因敲除的HEPG2细胞进行扩增和冻存。
| 货号 | EDC07758 |
|---|---|
| 产品名称 | CCR6基因敲除HEPG2细胞 |
| 物种 | 人 |
| 细胞 | HEPG2 |
| Cellosaurus ID | CVCL_0027 |
| 细胞别名 | HEP-G2, Hep G2, HEP G2, HepG2, HEPG2 |
| 消化时间 | 1.5 min~2 min |
| 基因 | CCR6 |
| 传代比例 | 01:03 |
| 基因ID | |
| 摘要 |
This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The gene is preferentially expressed by immature dendritic cells and memory T cells. The ligand of this receptor is macrophage inflammatory protein 3 alpha (MIP-3 alpha). This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses. Alternatively spliced transcript variants that encode the same protein have been described for this gene. [provided by RefSeq, Jul 2008]
|
| 癌症类型 | Hepatocellular Carcinoma |
| 细胞形态 | 贴壁生长 |
| 完全培养基 | DMEM+10% FBS |
| 冻存培养基 | 50% 基础培养基+40% FBS+10% DMSO |
* 仅供科研使用，不适用于人体或动物，包括临床、治疗或诊断用途。
| Loci | 送检细胞STR信息 送检细胞名: Hep-G2 | 细胞库细胞STR信息 细胞库细胞名: Hep-G2 | ||||
| Allele1 | Allele2 | Allele3 | Allele1 | Allele2 | Allele3 | |
| Amelogenin | X | Y | X | Y | ||
| CSF1P0 | 10 | 11 | 10 | 11 | ||
| D2S1338 | 19 | 20 | 19 | 20 | ||
| D3S1358 | 15 | 16 | 15 | 16 | ||
| D5S818 | 11 | 12 | 11 | 12 | ||
| D7S820 | 10 | 10 | ||||
| D8S1179 | 15 | 16 | 15 | 16 | ||
| D13S317 | 9 | 13 | 9 | 13 | ||
| D16S539 | 12 | 12 | 13 | |||
| D18S51 | 13 | 14 | 13 | 14 | ||
| D19S433 | 15.2 | 15.2 | ||||
| D21S11 | 29 | 31 | 29 | 31 | ||
| FGA | 22 | 25 | 22 | 25 | ||
| Penta D | 9 | 13 | 9 | 13 | ||
| Penta E | 15 | 20 | 15 | 20 | ||
| TH01 | 9 | 9 | ||||
| TPOX | 8 | 9 | 8 | 9 | ||
| vWA | 17 | 17 | ||||
| D6S1043 | 13 | |||||
| D12S391 | 21 | 25 | 21 | 25 | ||
| D2S441 | 11.3 | 14 | 11.3 | 14 | ||
| Amelogenin | X | Y | X | Y | ||
| CSF1PO | 10 | 11 | 10 | 11 | ||
| D251338 | 19 | 20 | 19 | 20 | ||
| D351358 | 15 | 16 | 15 | 16 | ||
| D55818 | 11 | 12 | 11 | 12 | ||
| D75820 | 10 | 10 | ||||
| D851179 | 15 | 16 | 15 | 16 | ||
| D135317 | 9 | 13 | 9 | 13 | ||
| D165539 | 12 | 12 | 13 | |||
| D18551 | 13 | 14 | 13 | 14 | ||
| D195433 | 15.2 | 15.2 | ||||
| D21511 | 29 | 31 | 29 | 31 | ||
| FGA | 22 | 25 | 22 | 25 | ||
| Penta D | 9 | 13 | 9 | 13 | ||
| Penta E | 15 | 20 | 15 | 20 | ||
| TH01 | 9 | 9 | ||||
| TPOX | 8 | 9 | 8 | 9 | ||
| vWA | 17 | 17 | ||||
| D651043 | 13 | |||||
| D125391 | 21 | 25 | 26 | 21 | 25 | |
| D2S441 | 11.3 | 14 | 11.3 | 14 | ||
* 该细胞系与收录于ATCC, DSMZ, JCRB 和 RIKEN数据库的细胞系STR数据匹配。
结论：该细胞 STR 鉴定正确。
结论：该细胞 STR 鉴定正确。
* 研究用途免责声明：本内容基于公开的研究数据、生物信息学资源及计算分析生成，仅供研究参考。
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