说明书 
艾迪基因基于自主研发的EditX™基因编辑平台，采用优化升级的CRISPR/Cas9系统，设计科学的CNR1基因敲除方案。通过RNP法、瞬转质粒法或慢病毒法将CRISPR/Cas9编辑体系递送到U251 MG细胞中，然后经过真核抗性标记筛选出阳性细胞池，再使用3D单细胞打印技术挑选单克隆细胞，最后通过基因组测序验证，检测合格后对CNR1基因敲除的U251 MG细胞进行扩增和冻存。
| 货号 | EDC07679 |
|---|---|
| 产品名称 | CNR1基因敲除U251-MG细胞 |
| 物种 | 人 |
| 细胞 | U251 MG |
| 消化时间 | 2 min |
| 基因 | CNR1 |
| 传代比例 | 01:03 |
| 基因ID | |
| 摘要 |
This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene. [provided by RefSeq, May 2009]
|
| 细胞形态 | 贴壁生长 |
| 完全培养基 | MEM+10% FBS+1% NEAA |
| 冻存培养基 | 95% 完全培养基+5% DMSO |
* 仅供科研使用，不适用于人体或动物，包括临床、治疗或诊断用途。
* 研究用途免责声明：本内容基于公开的研究数据、生物信息学资源及计算分析生成，仅供研究参考。
联系电话