MMP11基因敲除MIA Paca-2细胞
货号:
EDJ-KQ58
物种:
人
细胞名称:
MIA Paca-2
基因名称:
MMP11
基因ID:
4320
规格:
1×10⁶cells
艾迪基因基于自主研发的EditX™基因编辑平台，采用优化升级的CRISPR/Cas9系统，设计科学的MMP11基因敲除方案。通过RNP法、瞬转质粒法或慢病毒法将CRISPR/Cas9编辑体系递送到MIA Paca-2细胞中，然后经过真核抗性标记筛选出阳性细胞池，再使用3D单细胞打印技术挑选单克隆细胞，最后通过基因组测序验证，检测合格后对MMP11基因敲除的MIA Paca-2细胞进行扩增和冻存。
| 货号 | EDJ-KQ58 |
|---|---|
| 产品名称 | MMP11基因敲除细胞(MIA Paca-2) |
| Cellosaurus ID | CVCL_0428 |
| 基因 | MMP11 |
| 基因ID | |
| 基因别名 | SL-3|ST3|STMY3 |
| 摘要 |
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is activated intracellularly by furin within the constitutive secretory pathway. Also in contrast to other MMP's, this enzyme cleaves alpha 1-proteinase inhibitor but weakly degrades structural proteins of the extracellular matrix. [provided by RefSeq, Jul 2008]
|
| 癌症类型 | Pancreatic Carcinoma |
| 细胞形态 | 半贴壁半悬浮 |
| 传代比率 | 1:3-1:8 |
| 完全培养基 | 1640+10% FBS |
| 冻存培养基 | 95%完全培养基 + 5% DMSO(ATCC) |
* 仅供科研使用，不适用于人体或动物，包括临床、治疗或诊断用途。
| Loci | 送检细胞STR信息 送检细胞名: MIA Paca-2 | 细胞库细胞STR信息 细胞库细胞名: MIA Paca-2 | ||
| Allele1 | Allele2 | Allele1 | Allele2 | |
| Amelogenin | X | X | ||
| CSF1PO | 10 | 10 | ||
| D2S1338 | 25 | 25 | ||
| D3S1358 | 16 | 16 | ||
| D5S818 | 12 | 13 | 12 | 13 |
| D7S820 | 12 | 12 | ||
| D8S1179 | 16 | 16 | ||
| D13S317 | 12 | 13 | 12 | 13 |
| D16S539 | 10 | 13 | 10 | 13 |
| D18S51 | 12 | 12 | ||
| D19S433 | 15 | 15 | ||
| D21S11 | 29 | 31.2 | 29 | 31.2 |
| FGA | 22 | 22 | ||
| Penta D | 12 | 16 | 12 | 16 |
| Penta E | 13 | 18 | 13 | 18 |
| TH01 | 9 | 10 | 9 | 10 |
| TPOX | 9 | 9 | ||
| vWA | 15 | 15 | ||
| D6S1043 | 12 | 12 | ||
| D12S391 | 19 | 19 | ||
| D2S441 | 14 | 14 | ||
* 该细胞系与收录于ATCC, DSMZ, JCRB 和 RIKEN数据库的细胞系STR数据匹配。
结论：该细胞 STR 鉴定正确。
结论：该细胞 STR 鉴定正确。
* 研究用途免责声明：本内容基于公开的研究数据、生物信息学资源及计算分析生成，仅供研究参考。
联系电话