OTOP1基因敲除HEK293细胞

Ammonium (NH), a breakdown product of amino acids that can be toxic at high levels, is detected by taste systems of organisms ranging from C. elegans to humans and has been used for decades in vertebrate taste research. Here we report that OTOP1, a proton-selective ion channel expressed in sour (Type III) taste receptor cells (TRCs), functions as sensor for ammonium chloride (NHCl). Extracellular NHCl evoked large dose-dependent inward currents in HEK-293 cells expressing murine OTOP1 (mOTOP1), human OTOP1 and other species variants of OTOP1, that correlated with its ability to alkalinize the cell cytosol. Mutation of a conserved intracellular arginine residue (R292) in the mOTOP1 tm 6-tm 7 linker specifically decreased responses to NHCl relative to acid stimuli. Taste responses to NHCl measured from isolated Type III TRCs, or gustatory nerves were strongly attenuated or eliminated in an Otop1 mouse strain. Behavioral aversion of mice to NHCl, reduced in Skn-1a mice lacking Type II TRCs, was entirely abolished in a double knockout with Otop1. These data together reveal an unexpected role for the proton channel OTOP1 in mediating a major component of the taste of NHCl and a previously undescribed channel activation mechanism.