PHGDH基因敲除PK-15细胞
货号:
EDC08335
物种:
猪
细胞名称:
PK-15
基因名称:
PHGDH
基因ID:
100144529
规格:
1×10⁶cells
艾迪基因基于自主研发的EditX™基因编辑平台,采用优化升级的CRISPR/Cas9系统,设计科学的PHGDH基因敲除方案。通过RNP法、瞬转质粒法或慢病毒法将CRISPR/Cas9编辑体系递送到PK-15细胞中,然后经过真核抗性标记筛选出阳性细胞池,再使用3D单细胞打印技术挑选单克隆细胞,最后通过基因组测序验证,检测合格后对PHGDH基因敲除PK-15细胞进行扩增和冻存。
| 货号 | EDC08335 |
|---|---|
| 产品名称 | PHGDH基因敲除PK-15细胞 |
| 物种 | 猪 |
| 细胞 | PK-15 |
| Cellosaurus ID | CVCL_2160 |
| 细胞别名 | PK(15), PK (15), PK 15, PK15, Porcine Kidney-15 |
| 基因 | PHGDH |
| 消化时间 | 8-10 min |
| 基因ID | |
| 传代比例 | 1:3 |
| 摘要 |
This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known. [provided by RefSeq, Aug 2011]
|
| 细胞形态 | 贴壁生长 |
| 完全培养基 | DMEM + 10% FBS |
| 冻存培养基 | 92% 完全培养基 + 8% DMSO |
* 仅供科研使用,不适用于人体或动物,包括临床、治疗或诊断用途。
* 研究用途免责声明:本内容基于公开的研究数据、生物信息学资源及计算分析生成,仅供研究参考。