说明书 
艾迪基因基于自主研发的EditX™基因编辑平台，采用优化升级的CRISPR/Cas9系统，设计科学的PPM1A基因敲除方案。通过RNP法、瞬转质粒法或慢病毒法将CRISPR/Cas9编辑体系递送到HAP1细胞中，然后经过真核抗性标记筛选出阳性细胞池，再使用3D单细胞打印技术挑选单克隆细胞，最后通过基因组测序验证，检测合格后对PPM1A基因敲除的HAP1细胞进行扩增和冻存。
| 货号 | EDC07957 |
|---|---|
| 产品名称 | PPM1A基因敲除HAP1细胞 |
| 物种 | 人 |
| 细胞 | HAP1 |
| 细胞别名 | HAP-1 |
| 消化时间 | 2 min |
| 基因 | PPM1A |
| 传代比例 | 1:8~1:10 |
| 基因ID | |
| 摘要 |
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
|
| 细胞形态 | 贴壁生长 |
| 完全培养基 | IMDM+10%FBS |
| 冻存培养基 | 90%FBS+10%DMSO |
* 仅供科研使用，不适用于人体或动物，包括临床、治疗或诊断用途。
* 研究用途免责声明：本内容基于公开的研究数据、生物信息学资源及计算分析生成，仅供研究参考。
联系电话