PPP2R5E基因敲除HAP1细胞
货号:
EDC08318
物种:
人
细胞名称:
HAP1
基因名称:
PPP2R5E
基因ID:
5529
规格:
1×10⁶cells
艾迪基因基于自主研发的EditX™基因编辑平台,采用优化升级的CRISPR/Cas9系统,设计科学的PPP2R5E基因敲除方案。通过RNP法、瞬转质粒法或慢病毒法将CRISPR/Cas9编辑体系递送到HAP1细胞中,然后经过真核抗性标记筛选出阳性细胞池,再使用3D单细胞打印技术挑选单克隆细胞,最后通过基因组测序验证,检测合格后对PPP2R5E基因敲除HAP1细胞进行扩增和冻存。
| 货号 | EDC08318 |
|---|---|
| 产品名称 | PPP2R5E基因敲除HAP1细胞 |
| 物种 | 人 |
| 细胞 | HAP1 |
| 细胞别名 | HAP-1 |
| 基因 | PPP2R5E |
| 消化时间 | 1 min 30 s |
| 基因ID | |
| 传代比例 | 1:15-1:10 |
| 摘要 |
The protein encoded by this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an epsilon isoform of the regulatory subunit B56 subfamily. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2013]
|
| 细胞形态 | 贴壁生长 |
| 完全培养基 | IMDM + 10% FBS |
| 冻存培养基 | 90% FBS + 10% DMSO |
* 仅供科研使用,不适用于人体或动物,包括临床、治疗或诊断用途。
* 研究用途免责声明:本内容基于公开的研究数据、生物信息学资源及计算分析生成,仅供研究参考。