SBF1基因敲除293T细胞
货号:
EDJ-KQ157
物种:
人
细胞名称:
HEK293T
基因名称:
SBF1
基因ID:
6305
规格:
1×10⁶cells
艾迪基因基于自主研发的EditX™基因编辑平台，采用优化升级的CRISPR/Cas9系统，设计科学的SBF1基因敲除方案。通过RNP法、瞬转质粒法或慢病毒法将CRISPR/Cas9编辑体系递送到293T细胞中，然后经过真核抗性标记筛选出阳性细胞池，再使用3D单细胞打印技术挑选单克隆细胞，最后通过基因组测序验证，检测合格后对SBF1基因敲除的293T细胞进行扩增和冻存。
| 货号 | EDJ-KQ157 |
|---|---|
| 产品名称 | SBF1基因敲除细胞(293T) |
| Cellosaurus ID | CVCL_0063 |
| 细胞别名 | Hek293T, HEK-293T, HEK 293T, HEK-293-T, HEK 293 T, 293-T, 293 T, 293T, Human Embryonic Kidney 293T, 293tsA1609neo |
| 基因 | SBF1 |
| 基因ID | |
| 基因别名 | CMT4B3|DENND7A|MTMR5 |
| 摘要 |
This gene encodes a member of the protein-tyrosine phosphatase family. However, the encoded protein does not appear to be a catalytically active phosphatase because it lacks several amino acids in the catalytic pocket. This protein contains a Guanine nucleotide exchange factor (GEF) domain which is necessary for its role in growth and differentiation. Mutations in this gene have been associated with Charcot-Marie-Tooth disease 4B3. Pseudogenes of this gene have been defined on chromosomes 1 and 8. [provided by RefSeq, Dec 2014]
|
| 癌症类型 | Non-tumor |
| 细胞形态 | 贴壁 |
| 传代比率 | 1/5，2days |
| 完全培养基 | DMEM + 10% FBS + 1% NEAA+ 1% GlutaMax |
| 冻存培养基 | 95%完全培养基+ 5% DMSO |
* 仅供科研使用，不适用于人体或动物，包括临床、治疗或诊断用途。
| Loci | 送检细胞STR信息 送检细胞名: HEK293T | 细胞库细胞STR信息 细胞库细胞名: HEK293T | ||||
| Allele1 | Allele2 | Allele3 | Allele1 | Allele2 | Allele3 | |
| Amelogenin | X | X | ||||
| CSF1PO | 11 | 12 | 11 | 12 | ||
| D2S1338 | 19 | 19 | ||||
| D3S1358 | 15 | 16 | 17 | 15 | 16 | 17 |
| D5S818 | 8 | 9 | 8 | 9 | ||
| D7S820 | 11 | 11 | ||||
| D8S1179 | 11 | 12 | 14 | 12 | 14 | |
| D13S317 | 12 | 14 | 12 | 14 | ||
| D16S539 | 9 | 13 | 9 | 13 | ||
| D18S51 | 17 | 18 | 17 | 18 | ||
| D19S433 | 18 | 18 | ||||
| D21S11 | 28 | 30.2 | 28 | 30.2 | ||
| FGA | 23 | 23 | ||||
| Penta D | 9 | 10 | 9 | 10 | ||
| Penta E | 7 | 15 | 7 | 15 | ||
| TH01 | 7 | 9.3 | 7 | 9.3 | ||
| TPOX | 11 | 11 | ||||
| vWA | 16 | 19 | 16 | 19 | ||
| D6S1043 | 11 | |||||
| D12S391 | 19 | 21 | 19 | 21 | ||
| D2S441 | 11 | 15 | 11 | 15 | ||
* 该细胞系与收录于ATCC, DSMZ, JCRB 和 RIKEN数据库的细胞系STR数据匹配。
结论：该细胞 STR 鉴定正确。
结论：该细胞 STR 鉴定正确。
* 研究用途免责声明：本内容基于公开的研究数据、生物信息学资源及计算分析生成，仅供研究参考。
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