说明书 
艾迪基因基于自主研发的EditX™基因编辑平台，采用优化升级的CRISPR/Cas9系统，设计科学的UBE2B基因敲除方案。通过RNP法、瞬转质粒法或慢病毒法将CRISPR/Cas9编辑体系递送到HAP1细胞中，然后经过真核抗性标记筛选出阳性细胞池，再使用3D单细胞打印技术挑选单克隆细胞，最后通过基因组测序验证，检测合格后对UBE2B基因敲除HAP1细胞进行扩增和冻存。
| 货号 | EDC09501 |
|---|---|
| 产品名称 | UBE2B基因敲除HAP1细胞 |
| 物种 | 人 |
| 细胞 | HAP1 |
| 细胞别名 | HAP-1 |
| 消化时间 | 1 min 30 s |
| 基因 | UBE2B |
| 传代比例 | 1:15-1:10 |
| 基因ID | |
| 摘要 |
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq, Jul 2008]
|
| 细胞形态 | 贴壁生长 |
| 完全培养基 | IMDM + 10% FBS |
| 冻存培养基 | 90% FBS + 10% DMSO |
* 仅供科研使用，不适用于人体或动物，包括临床、治疗或诊断用途。
* 研究用途免责声明：本内容基于公开的研究数据、生物信息学资源及计算分析生成，仅供研究参考。
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