说明书 
艾迪基因基于自主研发的EditX™基因编辑平台，采用优化升级的CRISPR/Cas9系统，设计科学的FZD2基因敲除方案。通过RNP法、瞬转质粒法或慢病毒法将CRISPR/Cas9编辑体系递送到PANC-1细胞中，然后经过真核抗性标记筛选出阳性细胞池，再使用3D单细胞打印技术挑选单克隆细胞，最后通过基因组测序验证，检测合格后对FZD2基因敲除的PANC-1细胞进行扩增和冻存。
| 货号 | EDC07752 |
|---|---|
| 产品名称 | FZD2基因敲除PANC-1细胞 |
| 物种 | 人 |
| 细胞 | PANC-1 |
| Cellosaurus ID | CVCL_0480 |
| 细胞别名 | Panc-1, PANC.1, Panc 1, PanC1, Panc1, PANC1, Panc-1-P |
| 消化时间 | 3 min |
| 基因 | FZD2 |
| 传代比例 | 01:02 |
| 基因ID | |
| 摘要 |
This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. This gene encodes a protein that is coupled to the beta-catenin canonical signaling pathway. Competition between the wingless-type MMTV integration site family, member 3A and wingless-type MMTV integration site family, member 5A gene products for binding of this protein is thought to regulate the beta-catenin-dependent and -independent pathways. [provided by RefSeq, Dec 2010]
|
| 癌症类型 | Pancreatic Carcinoma |
| 细胞形态 | 贴壁生长 |
| 完全培养基 | DMEM+10% FBS |
| 冻存培养基 | 92% 完全培养液+8% DMSO |
* 仅供科研使用，不适用于人体或动物，包括临床、治疗或诊断用途。
| Loci | 送检细胞STR信息 送检细胞名: PANC-1 | 细胞库细胞STR信息 细胞库细胞名: PANC-1 | ||
| Allele1 | Allele2 | Allele1 | Allele2 | |
| Amelogenin | X | X | ||
| CSF1PO | 10 | 12 | 10 | 12 |
| D5S818 | 11 | 13 | 11 | 13 |
| D7S820 | 8 | 10 | 8 | 10 |
| D13S317 | 11 | 11 | ||
| D16S539 | 11 | 11 | ||
| TPOX | 8 | 11 | 8 | 11 |
| vWA | 15 | 15 | ||
* 该细胞系与收录于ATCC, DSMZ, JCRB 和 RIKEN数据库的细胞系STR数据匹配。
结论：该细胞 STR 鉴定正确。
结论：该细胞 STR 鉴定正确。
* 研究用途免责声明：本内容基于公开的研究数据、生物信息学资源及计算分析生成，仅供研究参考。
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