说明书 
艾迪基因基于自主研发的EditX™基因编辑平台，采用优化升级的CRISPR/Cas9系统，设计科学的SNCA基因敲除方案。通过RNP法、瞬转质粒法或慢病毒法将CRISPR/Cas9编辑体系递送到H4细胞中，然后经过真核抗性标记筛选出阳性细胞池，再使用3D单细胞打印技术挑选单克隆细胞，最后通过基因组测序验证，检测合格后对SNCA基因敲除的H4细胞进行扩增和冻存。
| 货号 | EDC07746 |
|---|---|
| 产品名称 | SNCA基因敲除H4细胞 |
| 物种 | 人 |
| 细胞 | H4 |
| 消化时间 | 2~3min |
| 基因 | SNCA |
| 传代比例 | 01:03 |
| 基因ID | |
| 摘要 |
Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016]
|
| 细胞形态 | 贴壁生长 |
| 完全培养基 | DMEM+10%FBS |
| 冻存培养基 | 55% 基础培养基+40%FBS+5%DMSO |
* 仅供科研使用，不适用于人体或动物，包括临床、治疗或诊断用途。
| Loci | 送检细胞STR信息 送检细胞名: H4 | 细胞库细胞STR信息 细胞库细胞名: H4 | ||
| Allele1 | Allele2 | Allele1 | Allele2 | |
| Amelogenin | X | Y | X | Y |
| CSF1PO | 10 | 12 | 10 | 12 |
| D2S1338 | 18 | 25 | 18 | 25 |
| D3S1358 | 17 | 18 | 17 | 18 |
| D5S818 | 10 | 12 | 10 | 12 |
| D7S820 | 8 | 11 | 8 | 11 |
| D8S1179 | 9 | 12 | 9 | 12 |
| D8S1179 | 14 | 14 | ||
| D13S317 | 12 | 12 | ||
| D16S539 | 11 | 12 | 11 | 12 |
| D18S51 | 14 | 16 | 14 | 16 |
| D19S433 | 12 | 14 | 12 | 14 |
| D21S11 | 30 | 31 | 30 | 31 |
| FGA | 19 | 25 | 19 | 25 |
| Penta D | 10 | 12 | 10 | 12 |
| Penta E | 5 | 12 | 5 | 12 |
| TH01 | 7 | 9 | 7 | 9 |
| TPOX | 8 | 11 | 8 | 11 |
| vWA | 14 | 18 | 14 | 18 |
* 该细胞系与收录于ATCC, DSMZ, JCRB 和 RIKEN数据库的细胞系STR数据匹配。
结论：该细胞 STR 鉴定正确。
结论：该细胞 STR 鉴定正确。
* 研究用途免责声明：本内容基于公开的研究数据、生物信息学资源及计算分析生成，仅供研究参考。
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