说明书 
艾迪基因基于自主研发的EditX™基因编辑平台，采用优化升级的CRISPR/Cas9系统，设计科学的TNFRSF1B基因敲除方案。通过RNP法、瞬转质粒法或慢病毒法将CRISPR/Cas9编辑体系递送到HAP1细胞中，然后经过真核抗性标记筛选出阳性细胞池，再使用3D单细胞打印技术挑选单克隆细胞，最后通过基因组测序验证，检测合格后对TNFRSF1B基因敲除的HAP1细胞进行扩增和冻存。
| 货号 | EDC08051 |
|---|---|
| 产品名称 | TNFRSF1B基因敲除HAP1细胞 |
| 物种 | 人 |
| 细胞 | HAP1 |
| 细胞别名 | HAP-1 |
| 消化时间 | 2 min |
| 基因 | TNFRSF1B |
| 传代比例 | 1:8~1:10 |
| 基因ID | |
| 摘要 |
The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. [provided by RefSeq, Jul 2008]
|
| 细胞形态 | 贴壁生长 |
| 完全培养基 | IMDM+10%FBS |
| 冻存培养基 | 90%FBS+10%DMSO |
* 仅供科研使用，不适用于人体或动物，包括临床、治疗或诊断用途。
* 研究用途免责声明：本内容基于公开的研究数据、生物信息学资源及计算分析生成，仅供研究参考。
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