CYBB基因敲除HL-60细胞
货号:
EDC08371
物种:
人
细胞名称:
HL-60
基因名称:
CYBB
基因ID:
1536
规格:
1×10⁶cells
艾迪基因基于自主研发的EditX™基因编辑平台，采用优化升级的CRISPR/Cas9系统，设计科学的CYBB基因敲除方案。通过RNP法、瞬转质粒法或慢病毒法将CRISPR/Cas9编辑体系递送到HL-60细胞中，然后经过真核抗性标记筛选出阳性细胞池，再使用3D单细胞打印技术挑选单克隆细胞，最后通过基因组测序验证，检测合格后对CYBB基因敲除HL-60细胞进行扩增和冻存。
| 货号 | EDC08371 |
|---|---|
| 产品名称 | CYBB基因敲除HL-60细胞 |
| 物种 | 人 |
| 细胞 | HL-60 |
| Cellosaurus ID | CVCL_0002 |
| 细胞别名 | HL 60, HL.60, HL60, Human Leukemia-60 |
| 基因 | CYBB |
| 消化时间 | / |
| 基因ID | |
| 传代比例 | 1:3 |
| 摘要 |
Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008]
|
| 细胞形态 | 悬浮生长 |
| 完全培养基 | IMDM + 20% FBS |
| 冻存培养基 | 92% 完全培养基 + 8% DMSO |
* 仅供科研使用，不适用于人体或动物，包括临床、治疗或诊断用途。
| Loci | 送检细胞STR信息 送检细胞名: HL-60 | 细胞库细胞STR信息 细胞库细胞名: HL-60 | ||
| Allele1 | Allele2 | Allele1 | Allele2 | |
| Amelogenin | X | X | ||
| CSF1PO | 13 | 14 | 13 | 14 |
| D2S1338 | 17 | 17 | ||
| D3S1358 | 16 | 16 | ||
| D5S818 | 12 | 12 | ||
| D7S820 | 11 | 12 | 11 | 12 |
| D8S1179 | 12 | 13 | 12 | 13 |
| D13S317 | 8 | 11 | 8 | 11 |
| D16S539 | 11 | 11 | ||
| D18S51 | 14 | 15 | 14 | 15 |
| D19S433 | 14 | 14 | ||
| D21S11 | 29 | 30 | 29 | 30 |
| FGA | 22 | 24 | 22 | 24 |
| Penta D | 10 | 12 | 10 | 12 |
| Penta E | 13 | 14 | 13 | 14 |
| TPOX | 8 | 11 | 8 | 11 |
| vWA | 16 | 16 | ||
* 该细胞系与收录于ATCC, DSMZ, JCRB 和 RIKEN数据库的细胞系STR数据匹配。
结论：该细胞 STR 鉴定正确。
结论：该细胞 STR 鉴定正确。
* 研究用途免责声明：本内容基于公开的研究数据、生物信息学资源及计算分析生成，仅供研究参考。
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