NR1H3基因敲除293T细胞
货号:
EDJ-KQ109
物种:
人
细胞名称:
HEK293T
基因名称:
NR1H3
基因ID:
10062
规格:
1×10⁶cells
艾迪基因基于自主研发的EditX™基因编辑平台，采用优化升级的CRISPR/Cas9系统，设计科学的NR1H3基因敲除方案。通过RNP法、瞬转质粒法或慢病毒法将CRISPR/Cas9编辑体系递送到293T细胞中，然后经过真核抗性标记筛选出阳性细胞池，再使用3D单细胞打印技术挑选单克隆细胞，最后通过基因组测序验证，检测合格后对NR1H3基因敲除的293T细胞进行扩增和冻存。
| 货号 | EDJ-KQ109 |
|---|---|
| 产品名称 | NR1H3基因敲除细胞(293T) |
| Cellosaurus ID | CVCL_0063 |
| 细胞别名 | Hek293T, HEK-293T, HEK 293T, HEK-293-T, HEK 293 T, 293-T, 293 T, 293T, Human Embryonic Kidney 293T, 293tsA1609neo |
| 基因 | NR1H3 |
| 基因ID | |
| 基因别名 | LXR-a|LXRA|RLD-1 |
| 摘要 |
The protein encoded by this gene belongs to the NR1 subfamily of the nuclear receptor superfamily. The NR1 family members are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. This protein is highly expressed in visceral organs, including liver, kidney and intestine. It forms a heterodimer with retinoid X receptor (RXR), and regulates expression of target genes containing retinoid response elements. Studies in mice lacking this gene suggest that it may play an important role in the regulation of cholesterol homeostasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
|
| 癌症类型 | Non-tumor |
| 细胞形态 | 贴壁 |
| 传代比率 | 1/5，2days |
| 完全培养基 | DMEM + 10% FBS + 1% NEAA+ 1% GlutaMax |
| 冻存培养基 | 95%完全培养基+ 5% DMSO |
* 仅供科研使用，不适用于人体或动物，包括临床、治疗或诊断用途。
| Loci | 送检细胞STR信息 送检细胞名: HEK293T | 细胞库细胞STR信息 细胞库细胞名: HEK293T | ||||
| Allele1 | Allele2 | Allele3 | Allele1 | Allele2 | Allele3 | |
| Amelogenin | X | X | ||||
| CSF1PO | 11 | 12 | 11 | 12 | ||
| D2S1338 | 19 | 19 | ||||
| D3S1358 | 15 | 16 | 17 | 15 | 16 | 17 |
| D5S818 | 8 | 9 | 8 | 9 | ||
| D7S820 | 11 | 11 | ||||
| D8S1179 | 11 | 12 | 14 | 12 | 14 | |
| D13S317 | 12 | 14 | 12 | 14 | ||
| D16S539 | 9 | 13 | 9 | 13 | ||
| D18S51 | 17 | 18 | 17 | 18 | ||
| D19S433 | 18 | 18 | ||||
| D21S11 | 28 | 30.2 | 28 | 30.2 | ||
| FGA | 23 | 23 | ||||
| Penta D | 9 | 10 | 9 | 10 | ||
| Penta E | 7 | 15 | 7 | 15 | ||
| TH01 | 7 | 9.3 | 7 | 9.3 | ||
| TPOX | 11 | 11 | ||||
| vWA | 16 | 19 | 16 | 19 | ||
| D6S1043 | 11 | |||||
| D12S391 | 19 | 21 | 19 | 21 | ||
| D2S441 | 11 | 15 | 11 | 15 | ||
* 该细胞系与收录于ATCC, DSMZ, JCRB 和 RIKEN数据库的细胞系STR数据匹配。
结论：该细胞 STR 鉴定正确。
结论：该细胞 STR 鉴定正确。
* 研究用途免责声明：本内容基于公开的研究数据、生物信息学资源及计算分析生成，仅供研究参考。
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