说明书 
艾迪基因基于自主研发的EditX™基因编辑平台，采用优化升级的CRISPR/Cas9系统，设计科学的RIPK1基因敲除方案。通过RNP法、瞬转质粒法或慢病毒法将CRISPR/Cas9编辑体系递送到HEK293T细胞中，然后经过真核抗性标记筛选出阳性细胞池，再使用3D单细胞打印技术挑选单克隆细胞，最后通过基因组测序验证，检测合格后对RIPK1基因敲除的HEK293T细胞进行扩增和冻存。
| 货号 | EDC08141 |
|---|---|
| 产品名称 | RIPK1基因敲除HEK293T细胞 |
| 物种 | 人 |
| 细胞 | HEK293T |
| Cellosaurus ID | CVCL_0063 |
| 细胞别名 | Hek293T, HEK-293T, HEK 293T, HEK-293-T, HEK 293 T, 293-T, 293 T, 293T, Human Embryonic Kidney 293T, 293tsA1609neo |
| 消化时间 | 30 sec~1 min |
| 基因 | RIPK1 |
| 传代比例 | 01:05 |
| 基因ID | |
| 摘要 |
This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein plays a role in inflammation and cell death in response to tissue damage, pathogen recognition, and as part of developmental regulation. RIPK1/RIPK3 kinase-mediated necrosis is referred to as necroptosis. Genetic disruption of this gene in mice results in death shortly after birth. [provided by RefSeq, Aug 2017]
|
| 癌症类型 | Non-tumor |
| 细胞形态 | 贴壁生长 |
| 完全培养基 | DMEM+10% FBS+1% NEAA+1% GlutaMax |
| 冻存培养基 | 95% 完全培养基+5% DMSO |
* 仅供科研使用，不适用于人体或动物，包括临床、治疗或诊断用途。
| Loci | 送检细胞STR信息 送检细胞名: HEK293T | 细胞库细胞STR信息 细胞库细胞名: HEK293T | ||||
| Allele1 | Allele2 | Allele3 | Allele1 | Allele2 | Allele3 | |
| Amelogenin | X | X | ||||
| CSF1PO | 11 | 12 | 11 | 12 | ||
| D2S1338 | 19 | 19 | ||||
| D3S1358 | 15 | 16 | 17 | 15 | 16 | 17 |
| D5S818 | 8 | 9 | 8 | 9 | ||
| D7S820 | 11 | 11 | ||||
| D8S1179 | 11 | 12 | 14 | 12 | 14 | |
| D13S317 | 12 | 14 | 12 | 14 | ||
| D16S539 | 9 | 13 | 9 | 13 | ||
| D18S51 | 17 | 18 | 17 | 18 | ||
| D19S433 | 18 | 18 | ||||
| D21S11 | 28 | 30.2 | 28 | 30.2 | ||
| FGA | 23 | 23 | ||||
| Penta D | 9 | 10 | 9 | 10 | ||
| Penta E | 7 | 15 | 7 | 15 | ||
| TH01 | 7 | 9.3 | 7 | 9.3 | ||
| TPOX | 11 | 11 | ||||
| vWA | 16 | 19 | 16 | 19 | ||
| D6S1043 | 11 | |||||
| D12S391 | 19 | 21 | 19 | 21 | ||
| D2S441 | 11 | 15 | 11 | 15 | ||
* 该细胞系与收录于ATCC, DSMZ, JCRB 和 RIKEN数据库的细胞系STR数据匹配。
结论：该细胞 STR 鉴定正确。
结论：该细胞 STR 鉴定正确。
* 研究用途免责声明：本内容基于公开的研究数据、生物信息学资源及计算分析生成，仅供研究参考。
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