β1integrin基因敲除HEPG2细胞
货号:
EDJ-KQ37
物种:
人
细胞名称:
Hep-G2
基因名称:
ITGB1
基因ID:
3688
规格:
1×10⁶cells
艾迪基因基于自主研发的EditX™基因编辑平台，采用优化升级的CRISPR/Cas9系统，设计科学的β1integrin基因敲除方案。通过RNP法、瞬转质粒法或慢病毒法将CRISPR/Cas9编辑体系递送到HEPG2细胞中，然后经过真核抗性标记筛选出阳性细胞池，再使用3D单细胞打印技术挑选单克隆细胞，最后通过基因组测序验证，检测合格后对β1integrin基因敲除的HEPG2细胞进行扩增和冻存。
| 货号 | EDJ-KQ37 |
|---|---|
| 产品名称 | β1integrin基因敲除细胞(HEPG2) |
| Cellosaurus ID | CVCL_0027 |
| 细胞别名 | HEP-G2, Hep G2, HEP G2, HepG2, HEPG2 |
| 基因 | β1integrin |
| 基因ID | |
| 摘要 |
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
|
| 细胞形态 | 贴壁 |
| 传代比率 | 1:2-1:3 |
| 完全培养基 | dmem+10%fbs |
| 冻存培养基 | 70%完培+20%fbs+10%dmso |
* 仅供科研使用，不适用于人体或动物，包括临床、治疗或诊断用途。
| Loci | 送检细胞STR信息 送检细胞名: Hep-G2 | 细胞库细胞STR信息 细胞库细胞名: Hep-G2 | ||
| Allele1 | Allele2 | Allele1 | Allele2 | |
| Amelogenin | X | Y | X | Y |
| CSF1P0 | 10 | 11 | 10 | 11 |
| D2S1338 | 19 | 20 | 19 | 20 |
| D3S1358 | 15 | 16 | 15 | 16 |
| D5S818 | 11 | 12 | 11 | 12 |
| D7S820 | 10 | 10 | ||
| D8S1179 | 15 | 16 | 15 | 16 |
| D13S317 | 9 | 13 | 9 | 13 |
| D16S539 | 12 | 12 | 13 | |
| D18S51 | 13 | 14 | 13 | 14 |
| D19S433 | 15.2 | 15.2 | ||
| D21S11 | 29 | 31 | 29 | 31 |
| FGA | 22 | 25 | 22 | 25 |
| Penta D | 9 | 13 | 9 | 13 |
| Penta E | 15 | 20 | 15 | 20 |
| TH01 | 9 | 9 | ||
| TPOX | 8 | 9 | 8 | 9 |
| vWA | 17 | 17 | ||
| D6S1043 | 13 | |||
| D12S391 | 21 | 25 | 21 | 25 |
| D2S441 | 11.3 | 14 | 11.3 | 14 |
* 该细胞系与收录于ATCC, DSMZ, JCRB 和 RIKEN数据库的细胞系STR数据匹配。
结论：该细胞 STR 鉴定正确。
结论：该细胞 STR 鉴定正确。
* 研究用途免责声明：本内容基于公开的研究数据、生物信息学资源及计算分析生成，仅供研究参考。
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