说明书 
艾迪基因基于自主研发的EditX™基因编辑平台，采用优化升级的CRISPR/Cas9系统，设计科学的PTGER4基因敲除方案。通过RNP法、瞬转质粒法或慢病毒法将CRISPR/Cas9编辑体系递送到RT4细胞中，然后经过真核抗性标记筛选出阳性细胞池，再使用3D单细胞打印技术挑选单克隆细胞，最后通过基因组测序验证，检测合格后对PTGER4基因敲除的RT4细胞进行扩增和冻存。
| 货号 | EDC07640 |
|---|---|
| 产品名称 | PTGER4基因敲除RT4细胞 |
| 物种 | 人 |
| 细胞 | RT4 |
| Cellosaurus ID | CVCL_0036 |
| 细胞别名 | RT4, RT4P |
| 消化时间 | 6 min |
| 基因 | PTGER4 |
| 传代比例 | 01:03 |
| 基因ID | |
| 摘要 |
The protein encoded by this gene is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). This receptor can activate T-cell factor signaling. It has been shown to mediate PGE2 induced expression of early growth response 1 (EGR1), regulate the level and stability of cyclooxygenase-2 mRNA, and lead to the phosphorylation of glycogen synthase kinase-3. Knockout studies in mice suggest that this receptor may be involved in the neonatal adaptation of circulatory system, osteoporosis, as well as initiation of skin immune responses. [provided by RefSeq, Jul 2008]
|
| 癌症类型 | Bladder Carcinoma |
| 细胞形态 | 贴壁生长 |
| 完全培养基 | McCOY's5A+10% FBS |
| 冻存培养基 | 92% 完全培养液+8% DMSO |
* 仅供科研使用，不适用于人体或动物，包括临床、治疗或诊断用途。
| Loci | 送检细胞STR信息 送检细胞名: RT-4 | 细胞库细胞STR信息 细胞库细胞名: RT-4 | ||
| Allele1 | Allele2 | Allele1 | Allele2 | |
| Amelogenin | X | Y | X | Y |
| CSF1PO | 10 | 12 | 10 | 12 |
| D2S1338 | 18 | 19 | 18 | 19 |
| D3S1358 | 15 | 15 | ||
| D5S818 | 11 | 12 | 11 | 12 |
| D7S820 | 9 | 12 | 9 | 12 |
| D8S1179 | 13 | 15 | 13 | 15 |
| D13S317 | 8 | 8 | ||
| D16S539 | 9 | 9 | ||
| D18S51 | 15 | 17 | 15 | 17 |
| D19S433 | 13 | 13 | ||
| D21S11 | 30 | 32.2 | 30 | 32.2 |
| FGA | 22 | 24 | 22 | 24 |
| Penta D | 12 | 13 | 12 | 13 |
| Penta E | 7 | 10 | 7 | 10 |
| TH01 | 9 | 9.3 | 9 | 9.3 |
| TPOX | 8 | 11 | 8 | 11 |
| vWA | 14 | 17 | 14 | 17 |
| D6S1043 | 13 | 19 | ||
| D12S391 | 20 | 22 | ||
| D2S441 | 10 | 13 | ||
* 该细胞系与收录于ATCC, DSMZ, JCRB 和 RIKEN数据库的细胞系STR数据匹配。
结论：该细胞 STR 鉴定正确。
结论：该细胞 STR 鉴定正确。
* 研究用途免责声明：本内容基于公开的研究数据、生物信息学资源及计算分析生成，仅供研究参考。
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