trpv6基因敲除CaCO-2细胞
货号:
EDJ-KQ09
物种:
人
细胞名称:
Caco-2
基因名称:
TRPV6
基因ID:
55503
规格:
1×10⁶cells
艾迪基因基于自主研发的EditX™基因编辑平台，采用优化升级的CRISPR/Cas9系统，设计科学的trpv6基因敲除方案。通过RNP法、瞬转质粒法或慢病毒法将CRISPR/Cas9编辑体系递送到CaCO-2细胞中，然后经过真核抗性标记筛选出阳性细胞池，再使用3D单细胞打印技术挑选单克隆细胞，最后通过基因组测序验证，检测合格后对trpv6基因敲除的CaCO-2细胞进行扩增和冻存。
| 货号 | EDJ-KQ09 |
|---|---|
| 产品名称 | trpv6基因敲除细胞(CaCO-2) |
| Cellosaurus ID | CVCL_0025 |
| 细胞别名 | CaCo-2, CACO-2, Caco 2, CACO 2, CACO2, CaCo2, CaCO2, Caco2, Caco-2/ATCC, Caco-II |
| 基因 | trpv6 |
| 基因ID | |
| 基因别名 | ABP/ZF|CAT1|CATL|ECAC2|HRPTTN|HSA277909|LP6728|ZFAB |
| 摘要 |
This gene encodes a member of a family of multipass membrane proteins that functions as calcium channels. The encoded protein contains N-terminal ankyrin repeats, which are required for channel assembly and regulation. Translation initiation for this protein occurs at a non-AUG start codon that is decoded as methionine. This gene is situated next to a closely related gene for transient receptor potential cation channel subfamily V member 5 (TRPV5). This locus has experienced positive selection in non-African populations, resulting in several non-synonymous codon differences among individuals of different genetic backgrounds. [provided by RefSeq, Feb 2015]
|
| 癌症类型 | Colorectal Carcinoma |
| 细胞形态 | 贴壁 |
| 传代比率 | 1/4，2~3days |
| 完全培养基 | DMEM + 10% FBS；MEM+NEAA+20% FBS（procell） |
| 冻存培养基 | 70%完全培养基+ 20% FBS+ 10% DMSO |
* 仅供科研使用，不适用于人体或动物，包括临床、治疗或诊断用途。
| Loci | 送检细胞STR信息 送检细胞名: Caco-2 | 细胞库细胞STR信息 细胞库细胞名: Caco-2 | ||||
| Allele1 | Allele2 | Allele3 | Allele1 | Allele2 | Allele3 | |
| Amelogenin | X | X | ||||
| CSF1PO | 11 | 11 | ||||
| D5S818 | 12 | 13 | 12 | 13 | ||
| D7S820 | 11 | 12 | 11 | 12 | ||
| D13S317 | 11 | 13 | 14 | 11 | 13 | 14 |
| D16S539 | 12 | 13 | 12 | 13 | ||
| TPOX | 9 | 11 | 9 | 11 | ||
| vWA | 16 | 18 | 16 | 18 | ||
* 该细胞系与收录于ATCC, DSMZ, JCRB 和 RIKEN数据库的细胞系STR数据匹配。
结论：该细胞 STR 鉴定正确。
结论：该细胞 STR 鉴定正确。
* 研究用途免责声明：本内容基于公开的研究数据、生物信息学资源及计算分析生成，仅供研究参考。
联系电话